Application

in Vivo, Imaging

General description

The Baseclick EdU In Vivo Kits provide a superior alternative to BrdU and [3H]thymidine assays for detection of cell proliferation in whole animals, showing no toxic effects. EdU (5-ethynyl-2’-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis. In contrast to BrdU assays, the EdU In Vivo Assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the EdU In Vivo Kits utilize click chemistry for detection in a variety of dye fluorescent readouts. Furthermore, the streamlined detection protocol reduces both the total number of steps and significantly decreases the total amount of time. The simple click chemistry detection procedure is complete within 30 minutes and is compatible with multiplexing for content and context-rich results.The in vivo kits are designed in a sense that you combine one of the three baseclick EdU cell proliferation kits such as:1. Imaging kit (BCK-IV-IM)2. Flow cytometry kit (BCK-IV-FC)3. High throughput screening – HTS kit (BCK-IV-HTS) You can select the above kits with the dye of choice and the right EdU content for your animal model. Depending on your animal model or the number of animals to be tested you can choose between three kit sizes S, M, and L with increasing EdU content, as indicated in the following table. Kit Size Content of EdU S 50 mg M 500 mg L 1000 mg